Heterogeneity in Colicin E2 expression of Escherichia coli

In a combined theoretical and experimental approach we plan to study heterogeneous gene expression of the Escherichia coli Colicin E2 operon in individual cells. Using fluorescence time-lapse microscopy we aim to quantitatively analyse the kinetics of Colicin expression from the Colicin operon and investigate further factors such as small RNAs regulating Colicin release and its impact on the establishment of heterogeneity. In particular, we are interested in changes of expression dynamics in dependence of SOS response and how this affects the amount and time-point of Colicin release.